Not all Calories are created equal.

I had always assumed (without thinking much about it) that when a food package claimed its contents represented 100 Calories then that figure would be basically correct. In other words, if you took the food and dessicated it and burned it, it would give off 100 Calories. I’d also assumed (even though if I’d thought about it I’d have known this to be wrong) that the amount of energy in the food would be closely correlated to the amount of energy the body could absorb from the food. Both assumptions turn out to be incorrect.

The first assumption is incorrect because the second one is incorrect. Foods are labeled using a process called the Atwater System which attempts to adjust the actual Calorie content of food by a factor related to how well the body absorbs energy from that food.

Note that in this essay I focus on the ability of various foods to provide energy (that is Calories, or at the lowest level ATP molecules), not on food’s structural (or other properties). I am interested in how best to refuel during a race — which means about all that matters is energy. This is not about recovery and only slightly about pre-race preparation.

Carbohydrates

Let’s start by looking at the monosaccharide, glucose. Eukaryot cells can directly catabolize it. Each glucose molecule goes through a series of reactions in which it is broken down into CO2 and water, in the process it releases (in optimal conditions) 38 ATP molecules. ATP molecules are then used to power reactions within the cell which require energy. In actual practice only about 28-30 ATP molecules are produced (conditions inside the cell are rarely optimal).

Now the energy released in ATP⇒ADP + P reaction is: 7.3kcal/mole. So, since the heat of combustion of a mole of glucose is 686Cal, then the body captures about 29*7.3/686 = 30.8% of this total energy. The rest is released as heat.

When not exercising glucose can be stored in muscles as glycogen and it takes 1 ATP molecule to add a glucose molecule to a glycogen one (Glycogenesis). The reverse reaction (Glycogenolysis) does not produce any ATP so storing glucose as glycogen represents a 3~4% energy loss. (But this loss will be irrelevant to someone exercising as it will not happen then).

Now let’s look at fructose, another monosaccharide, but not one that most cells of the human body can directly catabolize. Instead the liver snags fructose molecules and converts about half of them to glucose at a cost of 2 ATP molecules per fructose molecule. Some of the rest of the fructose is catabolized directly by the liver a process approximately as efficient as glucose catabolism. So efficiency of fructose catabolism is dependent on where the fructose gets used, but, averaging over both pathways, a molecule of fructose produces one fewer molecule of ATP than glucose does, and much of the energy will not reach the muscles at all.

A sucrose molecule consists of a glucose and a fructose molecule bound together. Joining the two involves releasing a molecule of water, so sucrose is slightly more energy dense than its components. In the gut sucrose is broken down into its two components, and their energy is then released as if they had been swallowed whole.

Not all carbohydrates are digestible (cellulose is not), but they will still burn at about 4Cal/gram in calorimeter, so all those Calories will be completely wasted to the body.

substance Cal/gram moles ATP/gram moles ATP/Cal
glucose 3.81 ~29/180 ≅ .161 .0423
fructose 3.79 ~28/180 ≅ .156 .0410
sucrose 3.94 ~57/342 ≅ .167 .0423
maltodextrin ~4.1 ~(n*29)/(n*172+18) ≅ .167 .0406
(maltodextrin is a polymer containing many glucose molecules which is the primary ingredient of most gels. I have used 10 glucose for computational purposes.)

Fats

Now fats (and oils and such like) consist of three fatty acid molecules bound together by a glycerol. They are broken down in the gut. Some can diffuse directly into the blood stream from there, while others go through a more complicated process. Fatty acids are not very soluble in water, but there is a protein in the blood (albumin) which binds to them, thus increasing the blood’s ability to transport them. Like glucose fatty acids can be used directly by the muscles. However because they are not very water soluble transporting them around the body and especially the cell takes longer than similar transport for glucose — which is why carbohydrates make a better fuel supply when energy is needed quickly. Moving a fatty acid into the mitocondria appears to be the rate limiting step for their catabolism.

A fatty acid consists of a COOH head (methyl, the acid) attached to a variable length carbon/hydrogen chain (the fat). In nature most chains are between 6 and 22 carbons long. Chains longer than 22 carbons are not catabolized efficiently. In saturated fats there are two hydrogens for each internal carbon, in unsaturated fats there is at least one C=C double bond. The energy in the molecule comes from the breakdown of the long chain. Every two atoms of saturated carbon on the chain yield theoretically 17, but in practice 14 molecules of ATP. For a fatty acid with 2n carbon atoms the total yield is about 14n-6 (as it requires some ATP to prepare the acid in the first place, and the final two carbons must be treated slightly differently).

Energy from fatty acids
fatty acid n ATP Δc mol weight ATP/g ATP/Cal
CH3(CH2)4COOH 3 36 835Cal 116 .310 .0431
CH3(CH2)14COOH 8 106 2390Cal 256 .414 .0444
CH3(CH2)20COOH 11 148 ??? 341 .434 ???
(Fatty acids with chains that are longer than this can be broken down, but no ATP will be generated until the chain is reduced to the above size)

Most (but not all) fatty acids in nature have an even number of carbon atoms in their chains. Odd numbers can be dealt in some animals, but no one is sure how (if) that works in humans. Unsaturated fats produce slightly less energy than saturated fats, the amount depending on whether the double bond is in an even or odd position in the chain and whether it is in a cis (more common in nature) or a trans (usually man-made) configuration — trans fats being more easily dealt with.

So, from the point of view of energy, saturated fats are better, and trans unsaturated fats are better than cis unsaturated fats. Rather the opposite of what we expect from a general health perspective.

Fat cannot easily be converted to glucose and so can’t be used to build glycogen stores.

Proteins

Proteins are broken into amino acids in the gut, and then processed further in the liver. But there are 20 amino acids that can be coded for to produce proteins and they have different properties. Most, but not all, can be decomposed to produce glucose, the others can be decomposed to make ketones which can be used in fatty acid catabolism, while some can go down either pathway.

When catabolizing carbohydrates and fats the waste products were just CO2 and water, which are easily disposed of. Amino acids contain all kinds of junk. All of them contain nitrogen which must be converted to urea. But converting nitrogen to urea costs ATP, about 4 of them per nitrogen atom.

Serine is a simple case: serine dehydratase converts serine to pyruvate and ammonia — without needing energy (ATP) input. Now pyruvate is an intermediate in the catabolism of glucose (in other words the cell can digest it). Two pyruvate molecules can be converted back to glucose (to be shipped out of the liver) at a cost of 4 ATP+2 GTP ≅ 6 ATP (Gluconeogenesis) or a pyruvate molecule can proceed down the Krebs cycle and eventually produce ~12 ATP, but that energy is stuck in the liver.

So if we want energy that can reach the muscles then two molecules of serine produce one molecule of glucose (at a cost of 6 ATP) and 2 molecules of urea (at a cost of 8 ATP) while glucose produces ~29ATP. So one serine molecule will produce ~(29-6-8)/2 = ~7.5 ATPs.

Alanine is another amino acid which can be converted to pyruvate.

Energy from amino acids
amino acid ATP Δc mol weight ATP/g ATP/Cal
serine 7.5 346Cal 105 .071 .0217

Most amino acids can be converted to glucose and so can be used to build glycogen stores — albeit inefficiently.

Unfortunately I have been unable to follow the metabolic pathways for other amino acids, so I don’t have a good handle of general protein catabolism.

Energy that is not directly used is lost as heat.

Conclusions

  • Per gram fats have between 2-2.5 times the useable energy of carbohydrates
  • Per gram carbohydrates have about twice the useable energy of serine
  • Catabolic efficiency (in terms of mole ATP/Calorie) is about the same for fats and carbohydrates
  • While serine is about half as useful as either.
  • In other words a Calorie of fat or carbohydrate is about twice as useful to the body as a Calorie of protein
  • Energy that is not directly used is lost as heat. Which means that using an inefficient fuel supply like protein will cause the body to heat up faster, which is generally not good.

Given these data, I don’t really see the point of the 40-30-30% mixture of a Balance Bar™ as an energy source during exercise. I see little reason to supply 30% protein, it just looks like a bad fuel supply to me (of course Balance Bars don’t say they are for exercisers). On the other hand it does explain why some GUs contain a little fat as well as carbs. Now an ultra runner, who needs energy at a slower rate than a marathon runner, should probably be eating more fat than a marathon runner…

Caveat: These results are the best I can do; I hope I have interpreted things accurately, but I am not a biochemist or a nutritionist.

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One Response to “Not all Calories are created equal.”

  1. Robin Taliaferro Says:

    George, It’s Robin Taliaferro. I found some old race results- some from the 90’s. Want them ? If yes, please email me your mailing address. My email: robint@cetstrategies.com

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